Rare Earth Element Biomining from the Great Salt Lake Brine Using Engineered E. Coli

This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions present. Our results showed that Tb binds to LBT, even in the presence of high concentrations of competing metals. We also tested REE adsorption at elevated temperatures (up to 100 degrees Celsius), and observed that Tb adsorption increases with temperature of to 70 degrees Celsius, and then remains constant until 100 degrees Celsius. Data analyses were performed using an ICP-MS at UCSC.

本数据集描述了经基因工程改造以表达镧系结合标签（lanthanide binding tag, LBT）的大肠杆菌（E. coli）细胞对稀土元素（rare earth element, REE）的吸附行为。实验以大盐湖合成溶液作为背景基质，将铽（Tb）的浓度设置为1~10000 ppb，该浓度远低于体系中共存的竞争离子浓度。研究结果表明，即便在高浓度共存竞争金属存在的条件下，铽仍可与LBT结合。此外，团队还在高温环境（最高达100摄氏度）下测试了稀土元素的吸附性能，观察到铽的吸附量随温度升高至70摄氏度时逐步提升，在70至100摄氏度区间内则保持稳定。所有数据分析工作均由加州大学圣克鲁兹分校（University of California, Santa Cruz, UCSC）的电感耦合等离子体质谱仪（Inductively Coupled Plasma Mass Spectrometry, ICP-MS）完成。