Gene expression profile of lung tissues comparing between ISM1-/- and wildtype mice.

Isthmin 1 (ISM1) is a secreted 60 kDa protein that exhibits pro-apoptotic and anti-angiogenic functions in experimental settings. We generated ISM1 knockout mice (ISM1-/- ) in C57BL/6J background through CRISPR gene editing technology to understand the physiological function of endogenous ISM1. The ISM1-/- mice presented spontaneous pulmonary emphysema and immune infiltration under the ambient environment. These findings suggest that ISM1 might be an anti-inflammatory protein important for maintaining lung homeostasis. To investigate the role of ISM1 in the lung under steady state, we compare the gene expression in the ISM1 knockout lungs with that of the wild type. Lungs of young adult mice (2 months old) from wild type and ISM1-/- mice were harvested and bulk RNASeq was performed. Total RNA extracted from whole lung tissue of ISM1-/- and WT mice at 2 months old in triplicates伊斯明1（Isthmin 1, ISM1）是一种分泌型60 kDa蛋白，在实验条件下可展现促凋亡与抗血管生成的功能。为阐明内源性ISM1的生理功能，本研究通过CRISPR基因编辑技术，在C57BL/6J遗传背景下构建了ISM1基因敲除小鼠（ISM1-/-）。在常规饲养环境中，ISM1-/-小鼠可自发出现肺气肿与免疫浸润表型。上述结果提示，ISM1或许是维持肺脏稳态的重要抗炎蛋白。为探究稳态条件下ISM1在肺脏中的作用，本研究将ISM1基因敲除小鼠肺脏的基因表达谱与野生型小鼠进行对比分析。本研究收集了2月龄年轻成年野生型与ISM1-/-小鼠的肺脏组织，开展了批量RNA测序（bulk RNASeq），并以3次生物学重复提取了2月龄ISM1-/-与野生型小鼠全肺组织的总RNA。