Verification of gene expression profiles in the liver tissues of mice after schistosomiasis infection

In order to further improve our method for measuring gene expression after different days of schistosomiasis infection, we adopted whole-genome microarray expression analysis as the sequencing platform to identify those genes with potential for distinguishing different infection times (applicable for the corresponding exposure range of medical decisions in cases of schistosomiasis infection after different days). We infected 15-day, 24-day and 45-day healthy mice with schistosomiasis and determined a consensus characteristic of successful schistosomiasis infection, which could distinguish infection days and control samples. Through real-time PCR, the expression of genes with significant differential expression (such as DDAH1) in the same RNA samples was quantified, confirming the low-level expression after infection and the expected schistosomiasis infection response pattern. In the liver tissues of mice, the gene expression changes caused by schistosome infection were measured at 0, 15, 24 and 45 days after infection. Three independent experiments were conducted at each time point, and different donor samples were used in each experiment.