Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning

O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome. Its dysregulation is associated with neurological disorders that impair cognitive function, and yet identification of phenotype-relevant candidate substrates in a brain-region-specific manner remains unfeasible. By combining an O-GlcNAc binding activity derived from Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling in Drosophila, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific candidate substrates enrichment. We mapped the O-GlcNAc interactome in major brain regions of Drosophila and found that components of the translational machinery, particularly ribosomal subunits, were abundantly O-GlcNAcylated in the mushroom body of Drosophila brain. Hypo-O-GlcNAcylation induced by ectopic expression of active CpOGA in the mushroom body decreased local translational activity, leading to ol..., , , # Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the *Drosophila* mushroom body contributing to olfactory learning

DOI:[10.5061/dryad.sj3tx969t ](https://doi.org/10.5061/dryad.sj3tx969t)

This dataset includes the data in the above publication. In this publication, we developed an *O*-GlcNAcylation profiling tool that translates *O*-GlcNAc modification into biotin conjugation for tissue-specific candidate substrate enrichment. We mapped the *O*-GlcNAc interactome in major brain regions of *Drosophila* and found that components of the translational machinery, particularly ribosomal subunits, were abundantly *O*-GlcNAcylated in the mushroom body of ...,

O-GlcNAc糖基化（O-GlcNAcylation）是一种使蛋白质组得以多样化的动态翻译后修饰。其失调与损害认知功能的神经系统疾病密切相关，但以脑区特异性方式鉴定表型相关的候选底物仍难以实现。研究人员将源自产气荚膜梭菌OGA（CpOGA）的O-GlcNAc结合活性，与果蝇（Drosophila）中的TurboID邻近标记技术相结合，开发出一款O-GlcNAc糖基化谱分析工具，该工具可将O-GlcNAc修饰转化为生物素缀合反应，从而实现组织特异性候选底物的富集。研究人员绘制了果蝇主要脑区的O-GlcNAc相互作用组，并发现在果蝇脑的蕈形体（mushroom body）中，翻译机器的组分尤其是核糖体亚基存在大量O-GlcNAc糖基化修饰。在蕈形体中异位表达活性CpOGA所诱导的O-GlcNAc糖基化不足，会降低局部翻译活性，进而导致……

# 数据来自：《组织特异性O-GlcNAc糖基化谱分析鉴定果蝇蕈形体翻译机器底物参与嗅觉学习》
DOI: 10.5061/dryad.sj3tx969t
https://doi.org/10.5061/dryad.sj3tx969t

本数据集包含上述发表文献中的相关数据。在该研究中，研究人员开发了一款O-GlcNAc糖基化谱分析工具，可将O-GlcNAc修饰转化为生物素缀合反应，以富集组织特异性候选底物。研究人员绘制了果蝇主要脑区的O-GlcNAc相互作用组，并发现在果蝇脑的蕈形体中，翻译机器的组分尤其是核糖体亚基存在大量O-GlcNAc糖基化修饰……