From oxidative stress to signal transduction: Adaptive mechanisms of Pseudomonas mosselii H6 to polyamide microplastics

As an emerging contaminant, microplastics have garnered widespread attention due to their ecological risks To examine the toxic effects of polyamide microplastics on microorganisms we investigated the mechanism of toxic effects of PAMPs at different concentrations and sizes on Pseudomonas mosselii H6 after a 48 h exposure test Results demonstrated that PAMPs had a significant concentration and size dependent inhibition on the biomass in biofilm of strain H6 and the highest inhibition rate of at the concentration of and the size of Meanwhile PAMPs could induced intracellular reactive oxygen species accumulation generated lipid peroxidation disrupted cell membrane integrity enhanced the release of intracellular lactate dehydrogenase and extracellular polymers and induced oxidative stress To resist the damage of PAMPs strain H6 upregulated the gene expression of primary metabolic pathways ABC transporter and activates two-component signaling systems to maintain cellular homeostasis This research illuminates the toxic effects and molecular mechanism of PAMPs on Pseudomonas mosselii H6 providing significant insights for risk assessment and management of microplastics in the environment