Soybean event DP-305423-1 and compositions and methods for the identification and/or detection thereof

Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically， the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8， 9， 14， 15， 20， 21， 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification， detection， and use of the soybean DP-305423-1 events are provided.

本发明提供了与转基因高油酸/ALS抑制剂耐受型大豆植株相关的组合物与方法。具体而言，本发明提供了携带DP-305423-1转化事件的大豆植株，该转化事件可赋予植株高油酸表型以及对至少一种ALS抑制剂类除草剂的耐受性。携带DP-305423-1转化事件的大豆植株，其基因组/转基因侧翼连接序列至少包含SEQ ID NO:8、9、14、15、20、21、83或84所示的多核苷酸序列。对DP-305423-1转化事件的基因组插入位点进行表征，可提升育种效率，并可借助分子标记追踪育种群体及其后代中的转基因插入片段。本发明同时提供了用于鉴定、检测以及应用DP-305423-1型大豆转化事件的多种方法与组合物。