Additional file 1: of ErbB4 promotes malignant peripheral nerve sheath tumor pathogenesis via Ras-independent mechanisms

Figure S1. ErbB4 lysates are sensitive to denaturing detergents. Table S1. Patient demographics for immunostained MPNSTs. Figure S2 Real-time PCR analyses of erbB4 splice variants and mouse MPNST erbB4 expression. Table S2. MPNST locations in P0-GGFβ3;Trp53+/â ;Erbb4flox/flox mice. Figure S3. Validation of Erbb4 ablation and effects on expression of other erbB receptors. Figure S4. Kinase array blots for NRG1β and unstimulated control and Erbb4-null MPNST cells. Figure S5. Downregulation of WNK1 expression did not affect cell viability. (ZIP 7528 kb)

图S1. ErbB4裂解液对变性去污剂敏感。
表S1. 免疫染色恶性周围神经鞘膜瘤（MPNST）的患者人口统计学资料。
图S2. erbB4剪接变体与小鼠恶性周围神经鞘膜瘤erbB4表达的实时聚合酶链反应（Real-time PCR）分析。
表S2. P0-GGFβ3;Trp53+/−;Erbb4flox/flox 小鼠的恶性周围神经鞘膜瘤发生部位。
图S3. Erbb4敲除验证及其对其他erbB受体表达的影响。
图S4. NRG1β刺激组、未刺激对照组及Erbb4敲除型恶性周围神经鞘膜瘤细胞的激酶阵列印迹。
图S5. WNK1表达下调对细胞活力无影响。（ZIP压缩文件，7528 KB）