Effect of pancreatin on acute pancreatitis resulting from L-arginine administration in mice, a morpho-histopathological and biochemical study

Abstract Acute pancreatitis (AP) is a life-unpleasant situation with contradictory and inadequate treatments. In this regard, the present study evaluated the effect of the possible pretreatment of lipase-pancreatin on L-arginine-induced AP. Forty adult mice were selected and divided into five groups: I) control group, II and III) AP groups (i.p.) receiving L-arginine of 2×300 and 2×400 mg/100 g body weight (b.w.), IV) AP (2×300 L-arginine) group + pancreatin (mice were i.p. injected by 350 U-lipase), and V) AP (2×400 L-arginine) group + pancreatin (mice were i.p. injected by 350 U-lipase). All AP groups displayed a significant increase in serum levels of ALT, AST, TBARS, and TNF-alpha compared to the control group. Moreover, pancreatic tissue edema, inflammation, and vacuolization of acinar cells were significantly higher in the untreated L-arginine group compared to the control and pancreatin groups. Conversely, the diameter of pancreatic islets significantly declined after induction of pancreatitis compared with control and pancreatin groups. Pancreatin treatment can be used in pancreatic dysfunction, however, this medicine showed no protective effect against L-arginine-induced AP in the mouse model.

摘要：急性胰腺炎（Acute pancreatitis, AP）是一种危及生命的不良病症，其治疗方案存在争议且疗效欠佳。本研究评估了脂肪酶-胰酶预处理对L-精氨酸诱导的急性胰腺炎的作用效果。选取40只成年小鼠并分为5组：I）对照组；II、III）AP模型组，分别经腹腔注射2×300 mg/100 g体重及2×400 mg/100 g体重的L-精氨酸；IV）AP（2×300 mg/100g L-精氨酸）+胰酶干预组，小鼠经腹腔注射350 U脂肪酶；V）AP（2×400 mg/100g L-精氨酸）+胰酶干预组，小鼠经腹腔注射350 U脂肪酶。所有AP模型组小鼠的血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、硫代巴比妥酸反应物（TBARS）及肿瘤坏死因子α（TNF-α）水平均较对照组显著升高。此外，未接受胰酶干预的L-精氨酸诱导AP小鼠的胰腺组织水肿、炎症反应及腺泡细胞空泡变性程度均显著高于对照组与胰酶干预组。与之相反，胰腺炎造模后小鼠胰岛直径较对照组及胰酶干预组显著降低。尽管胰酶可用于胰腺功能障碍的治疗，但本研究结果显示，在小鼠模型中，胰酶预处理并未对L-精氨酸诱导的急性胰腺炎展现出保护作用。