RNA Sequencing Facilitates Quantitative Analysis of shL3MBTL2 ,CGA-OE and corresponding control Transcriptomes

L3MBTL2 is a crucial component of ncPRC1.6 and has been implicated in transcriptional repression and chromatin compaction. However, the repression mechanism of L3MBTL2 and its biological functions are largely undefined. CGA encodes the alpha subunit of glycoprotein hormones, however, we showed that CGA plays an individual tumor suppressor role in PDAC. The goals of this part study are to compare transcriptome profiling of PANC-1-shL3MBTL2 cells or PANC-1-CGA-OE cells to their corresponding control cells. By identifying differentially expressed genes (DEGs) and enrichment pathways, the mechanism of L3MBTL2 and CGA in pancreatic cancer can be better studied. mRNA profiles of PANC-1 cells transfected with shCtrl, shL3MBTL2, vector or CGA-OE.