METHODS AND COMPOSITIONS FOR INTEGRATION OF AN EXOGENOUS SEQUENCE WITHIN THE GENOME OF PLANTS

Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes. The present disclosure provides methods and compositions for precision transformation， gene targeting， targeted genomic modification and protein expression in plants. In particular， the present disclosure describes a novel， transgenic marker-free strategy for integrating an exogenous sequence and to stack traits that exploit differential selection at an endogenous locus， e.g.， the acetohydroxyacid synthase (AHAS) locus) in plant genomes.

本文公开了用于植物中并行或顺序式转基因堆叠（transgene stacking）的方法与组合物，以获得具有选定表型的植物。本公开提供了用于植物的精准转化（precision transformation）、基因靶向（gene targeting）、靶向基因组修饰（targeted genomic modification）以及蛋白质表达（protein expression）的方法与组合物。具体而言，本公开描述了一种新型无标记转基因策略，可用于整合外源序列（exogenous sequence），并堆叠可利用植物基因组内源位点（endogenous locus）处差异筛选的性状，例如植物基因组中的乙酰羟酸合酶（acetohydroxyacid synthase, AHAS）位点。