Molecular characterization of the interneurons in human temporal neocortex by two photon fluorescence microscopy (v1.1)

The aim of this work is to study the 3D organization of some population of interneurons within a sample of interest, previously analyzed for physiological information, using two photon fluorescence microscopy (TPFM). We exploit the high axial and radial resolution of TPFM optical sectioning (0.44 x 0.44 x 2 μm³) in combination with a protocol for tissue clearing and labeling to perform the 3D reconstruction of 300 um thick brain sections. We clear the sections with the SWITCH/TDE clearing method and label the samples with three antibodies to co-stain three different populations of inhibitory interneurons: PV (Parvalbumin), SST(Somatostatin), and VIP (Vaso Intestinal Peptide). A previous data version of “Molecular characterization of the interneurons in human temporal neocortex by two photon fluorescence microscopy” can be found here: Costantini et al. (2020) [Data set, v1.0] [DOI: 10.25493/V9GW-4JG]( https://doi.org/10.25493%2FV9GW-4JG)

本研究旨在借助双光子荧光显微镜（two photon fluorescence microscopy, TPFM），对此前已开展生理学信息分析的目标样本中的特定中间神经元群的三维组织结构展开研究。本研究利用TPFM光学切片技术的高轴向、径向分辨率（0.44 × 0.44 × 2 μm³），结合组织透明化与标记方案，对厚度为300微米的脑切片进行三维重建。本研究采用SWITCH/TDE透明化方法处理切片，并使用三种抗体对样本进行共标记，以同时标记三类不同的抑制性中间神经元群：小白蛋白（Parvalbumin, PV）、生长抑素（Somatostatin, SST）以及血管活性肠肽（Vaso Intestinal Peptide, VIP）。本数据集的早期版本《基于双光子荧光显微镜的人类颞叶新皮层中间神经元分子特征解析》可通过以下途径获取：Costantini等人（2020）[数据集，v1.0] [DOI: 10.25493/V9GW-4JG]（https://doi.org/10.25493%2FV9GW-4JG）