The raw data of transcriptomes in whole brains when EcR-A was knocked down in pC1 neurons or not

The establishment of neural circuits for female sexual receptivity is essential to reproduction, but the underlying mechanism of associated neurodevelopment is still not fully understood. Here we found that neuropeptide prothoracicotropic hormone (PTTH), hormone of the PG axis, regulates virgin female receptivity through ecdysone in Drosophila melanogaster. PTTH-positive PG neurons are doublesex-expressing neurons, they regulate virgin female receptivity before the metamorphosis during the 3rd-instar larval stage. Furthermore, the genetic knocking down of ecdysone receptor EcR-A in pC1 neurons regulates the morphological development of pC1 neurons and further the virgin female copulation rate. However, the activity of pC1 neurons did not change, suggesting the changes of synaptic connection between pC1 neurons and other neurons. Analysis of brain transcriptomes when EcR-A was knocked down in pC1 neurons reveals that additional genes are regulated downstream of EcR-A in pC1 neurons. Our ..., ,

雌性性接受性神经环路的建立对繁殖至关重要，但其相关神经发育的潜在机制仍未完全阐明。本研究发现，作为前胸腺轴（PG axis）激素的神经肽促前胸腺激素（prothoracicotropic hormone, PTTH），可在黑腹果蝇（Drosophila melanogaster）中通过蜕皮激素调控处女雌蝇的性接受性。PTTH阳性前胸腺神经元为表达双性基因（doublesex）的神经元，它们在三龄幼虫期的变态发育前，调控处女雌蝇的性接受性。进一步研究表明，在pC1神经元中敲低蜕皮激素受体EcR-A，可调控pC1神经元的形态发育，进而影响处女雌蝇的交配率。然而pC1神经元的活性并未发生改变，这提示pC1神经元与其他神经元之间的突触连接发生了变化。对pC1神经元中EcR-A被敲低后的果蝇脑转录组进行分析，结果显示存在更多基因受pC1神经元内EcR-A的下游调控。本研究……