Whole genome sequencing of RPE1 LATS1/2-double knockout cells implanted into mice.

To demonstrate in vivo that DNA damage is induced in YAP-activated cells by weight-bearing activities, we performed whole genome DNA sequencing of RPE1 LATS1/2-double knockout cells were simultaneously implanted into the flank and footpad of Foxn1-null mice. To prevent cell death due to DNA damage, TP53 siRNA-loaded liposomes were injected into the developing tumor mass. Tumors were developed for 2 weeks after 4 days of wheel running (2 hrs per day). The genomic DNA sequence of RPE1 LATS1/2-double knockout cells cells transfected with TP53 siRNA was used as a reference.