Additional file 1 of Genome of the pincer wasp Gonatopus flavifemur reveals unique venom evolution and a dual adaptation to parasitism and predation

Additional file 1: Supplementary Table 1. Statistics of Nanopore sequencing. Supplementary Table 2. Statistics of Illumina sequencing. Supplementary Table 3. Statistics of Gonatopus flavifemur genome assembly by Nanopore reads. Supplementary Table 4. Statistics of Gonatopus flavifemur genome assembly after error-corrected by Illumina and Nanopore reads. Supplementary Table 5. Genome size of G. flavifemur estimated by flow cytometry and 17-mer analysis. Supplementary Table 6. The mapping rate with short sequencing reads of Gonatopus flavifemur genome. Supplementary Table 7. Genes in detoxification system and chemoreception system in 13 hymenopterans. Supplementary Table 8. TE sequences in Gonatopus flavifemur and other surveyed species. Supplementary Table 9. Characteristics of DNA TEs in Gonatopus flavifemur. Supplementary Table 10. GO enrichment analysis of Aculeate-specific genes, Biological Process category (padj < 0.05). Supplementary Table 11. GO enrichment analysis of Gonatopus flavifemur special genes. (padj < 0.05). Supplementary Table 12. Genes under positive selection in Gonatopus flavifemur detected by both aBSREL model in HyPhy and branch-site model in PAML. Supplementary Table 13. GO enrichment analysis of Gonatopus flavifemur expanded gene families, Biological Process category (padj < 0.05). Supplementary Table 14. Venom gland-associated genes (VGGs) in Gonatopus flavifemur. Supplementary Table 15. GO enrichment analysis of VGGs in Gonatopus flavifemur, Biological Process category (padj < 0.05). Supplementary Table 16. Chitinase-like genes in Gonatopus flavifemur and other species. Supplementary Table 17. Neprilysin-like genes in Gonatopus flavifemur and other Hymenoptera genomes. Supplementary Table 18. Upregulated genes in the prey-feeding female transcriptomes compared to the sucrose-feeding female transcriptomes. Supplementary Table 19. Yellow genes and expression level in Gonatopus flavifemur. Supplementary Table 20. Downregulated genes in the prey-feeding female transcriptomes compared to the sucrose-feeding female transcriptomes. Supplementary Table 21. GO enrichment analysis of extremely female-biased genes in Gonatopus flavifemur. Biological Process category (padj < 0.05). Supplementary Table 22. GO enrichment analysis of extremely male-biased genes in Gonatopus flavifemur. Biological Process category (padj < 0.05). Supplementary Table 23. Extremely male-biased genes in Gonatopus flavifemur. Supplementary Table 24. Sample information for sequencing used in this study.

附加文件1：补充表1 纳米孔（Nanopore）测序统计数据。补充表2 伊卢米纳（Illumina）测序统计数据。补充表3 基于纳米孔（Nanopore）读长的黄足螯蜂（Gonatopus flavifemur）基因组组装统计数据。补充表4 经伊卢米纳（Illumina）与纳米孔（Nanopore）读长纠错后的黄足螯蜂（Gonatopus flavifemur）基因组组装统计数据。补充表5 经流式细胞术（flow cytometry）与17聚体（17-mer）分析估算的黄足螯蜂（G. flavifemur）基因组大小。补充表6 黄足螯蜂（Gonatopus flavifemur）基因组的短测序读长比对率统计。补充表7 13种膜翅目昆虫解毒系统与化学感受系统相关基因统计。补充表8 黄足螯蜂（Gonatopus flavifemur）及其他调研物种的转座因子（Transposable Element, TE）序列信息。补充表9 黄足螯蜂（Gonatopus flavifemur）DNA转座因子（DNA TEs）的特征信息。补充表10 针尾亚目特异性基因的基因本体（Gene Ontology, GO）富集分析结果（生物过程类别，校正后P值<0.05）。补充表11 黄足螯蜂（Gonatopus flavifemur）特有基因的基因本体（GO）富集分析结果（校正后P值<0.05）。补充表12 同时通过HyPhy软件的aBSREL模型与PAML软件的分支位点模型检测到的黄足螯蜂（Gonatopus flavifemur）正选择基因。补充表13 黄足螯蜂（Gonatopus flavifemur）扩张基因家族的基因本体（GO）富集分析结果（生物过程类别，校正后P值<0.05）。补充表14 黄足螯蜂（Gonatopus flavifemur）毒腺相关基因（VGGs）。补充表15 黄足螯蜂（Gonatopus flavifemur）毒腺相关基因的基因本体（GO）富集分析结果（生物过程类别，校正后P值<0.05）。补充表16 黄足螯蜂（Gonatopus flavifemur）及其他物种的几丁质酶样基因。补充表17 黄足螯蜂（Gonatopus flavifemur）及其他膜翅目基因组中的脑啡肽酶样基因。补充表18 取食猎物的雌蜂转录组相较于取食蔗糖的雌蜂转录组的上调基因。补充表19 黄足螯蜂（Gonatopus flavifemur）的黄色基因（Yellow基因家族）及其表达水平。补充表20 取食猎物的雌蜂转录组相较于取食蔗糖的雌蜂转录组的下调基因。补充表21 黄足螯蜂（Gonatopus flavifemur）极端雌性偏态基因的基因本体（GO）富集分析结果（生物过程类别，校正后P值<0.05）。补充表22 黄足螯蜂（Gonatopus flavifemur）极端雄性偏态基因的基因本体（GO）富集分析结果（生物过程类别，校正后P值<0.05）。补充表23 黄足螯蜂（Gonatopus flavifemur）极端雄性偏态基因。补充表24 本研究所用测序样本的样本信息。