Temporal changes in (Fat-Mass and Weight) as measured by Dual Energy X-ray Absorptiometry (DEXA) for wild type and HYP mice infused with vehicle or SPR4-peptide (See Figure 7 for static changes).

DEXA measurements using a Lunar PIXImus system were carried out as described previously and discussed in methods [140]. Values are means (± SEM) of percentage changes that occurred from the start of the experiment (day 1; prior to pump implantation) to the end of the experiment (28 days later). Specifically, two measurements were taken, one on day 1 (baseline) and one on day 28. Mice were sacrificed on day 28 (age 9 weeks at sacrifice). Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice, SPR4 = infused SPR4-peptide and Vehicle = Saline infused. Unpaired t tests with confidence intervals of 95% were used for statistical analysis. Superscript letters (a, b or c) added to the calculated values indicate; a = significantly different to WT vehicle (WT-VE) infused mice pWT-VE versus WT-SPR4, HYP-VE versus HYP-SPR4 and WT-VE versus HYP-VE are shown in columns 3, 6 and 7 as indicated in the headings. In these columns (3, 6 and 7), the numbers that are significantly different (pIndex:%Δ-Fat = percentage change in fat-mass over 4 weeks; %Δ-Wgt = percentage change in weight over 4 weeks; %Δ-Fat/%Δ-Wgt = ratio of the changes in fat-mass and weight over 4 weeks.