Transcription profiling by array of skeletal and cardiac muscle from dysferlin-deficient mice

Dysferlin is expressed in skeletal and cardiac muscle. However, dysferlin deficiency, namely limb girdle muscular dystrophy 2B (LGMD2B) and Myoshi myopathy, results in skeletal muscle weakness and spares the heart. This dichotomy could be caused by differential regulation of protective mechanisms. Therefore, we compared intra-individual mRNA expression profiles between cardiac and skeletal muscle in dysferlin-deficient SJL/J mice and normal C57BL/6 mice. Experiment Overall Design: 20 chips were analyzed. They represent 4 groups of 5 replicates each. Experiment Overall Design: The 4 groups are cardiac (LV) and skeletal muscle of normal and dysferlin deficient mice. Experiment Overall Design: Tissues from normal mice are the controls in comparison to tissues of dysferlin deficient mice.dysferlin在骨骼肌与心肌中均有表达。然而，dysferlin缺陷可引发肢带型肌营养不良2B（limb girdle muscular dystrophy 2B, LGMD2B）与三宅肌病（Miyoshi myopathy），此类疾病仅导致骨骼肌无力，不会累及心脏。这种表型二分性可能由保护机制的差异调控所导致。因此，本研究对dysferlin缺陷型SJL/J小鼠与正常C57BL/6小鼠的心肌与骨骼肌的个体内mRNA表达谱进行了比较分析。 实验整体设计：共分析20张基因芯片。该20张芯片分为4组，每组设5个生物学重复。 实验整体设计：4组样本分别来自正常小鼠与dysferlin缺陷小鼠的心肌（左心室，LV）及骨骼肌。 实验整体设计：以正常小鼠的组织作为对照，与dysferlin缺陷小鼠的组织进行对比研究。