Sirtuin assay of purified Hos2 preparations using resveratrol

Fluorimetric Sirtuin1 like deacetylase assay with rHos2 protein using Fluor de Lys®−Sirt1 substrate in presence of Sirtuin1 activator Reserveratrol. This experiment is carried out in presence of HDAC inhibitor Trichostatin to inhibit any residual Histone deacetylase activity.

本实验以重组Hos2蛋白（rHos2 protein）为受试对象，采用Fluor de Lys®−Sirt1底物（Fluor de Lys®−Sirt1 substrate），在沉默信息调节因子1（Sirtuin1）激活剂白藜芦醇（Reserveratrol）存在的反应体系中，开展类Sirtuin1脱乙酰酶荧光检测实验。为抑制所有残留的组蛋白脱乙酰酶活性，本实验同时添加了组蛋白脱乙酰酶（HDAC）抑制剂曲古抑菌素（Trichostatin）。