Microwave-assisted extraction and content determination of astilbin in Lysiphyllum strychnifolium stems

Abstract Lysiphyllum strychnifolium (Craib) A. Schmitz. (in Thai name, Ya nang daeng) has been traditionally used to treat fever, alcohol intoxication, cancer, allergies, and blood toxins. It can be used as a health-promoting herbal tea and contains hydroalcoholic extracts. The purpose of the present study was to develop a microwave-assisted extraction method for astilbin in L. strychnifolium stems. HPLC was used to determine astilbin content. Three extraction conditions were optimized: types of solvent, microwave power levels, and the number of extraction cycles. Water:methanol (40:60) was the best solvent for astilbin extraction from L. strychnifolium stems using 450 watts and six microwave-assisted extraction cycles. This technique offers important advantages over conventional methods, such as shorter extraction times, substantial energy savings, and a reduced environmental burden.

摘要：Lysiphyllum strychnifolium (Craib) A. Schmitz.（泰语名：Ya nang daeng）在传统应用中常用于治疗发热、酒精中毒、癌症、过敏及血液毒素相关病症。该植物可作为保健凉茶使用，且含有水醇提取物。本研究旨在开发一种从L. strychnifolium茎中提取落新妇苷（astilbin）的微波辅助提取方法。采用高效液相色谱法（HPLC）对落新妇苷的含量进行测定。本研究对三项提取条件进行了优化：溶剂类型、微波功率及提取循环次数。最终确定的最优提取方案为：以水-甲醇（体积比40:60）作为提取溶剂，微波功率设置为450瓦，执行6次微波辅助提取循环。相较于传统提取方法，该技术具有提取时长更短、能源消耗大幅降低以及环境负担更小等显著优势。