Supplementary Materials for manuscript “Comparative genomics of three non-hematophagous leeches (<i>Whitmania</i> spp.): focusing on antithrombotic genes”

Leeches are well known for their blood-feeding habits and are widely used for medicinal purposes as they secrete various antithrombotic substances. However, some leeches such as <i>Whitmania</i> spp. exhibit non-hematophagous feeding habits and their significance for medicinal use is debated. Here, we provide two nearly complete high quality genomes of <i>Whitmania acranulata</i> and <i>Whitmania laevis</i>. We also identified many antithrombic gene families involved in anticoagulation, anti-platelet aggregation, fibrinolysis, and drug diffusion. Our results will greatly facilitate the evolutionary research and application of leech derivatives for medical and pharmaceutical purposes of thrombosis.File_S1_Wacr.genome.zip: Supplementary File S1, the genome assembly of W. acranulata.File_S2_Wlae.genome.zip: Supplementary File S2, the genome assembly of W. laevis.File_S3_Wacr.gff.zip: Supplementary File S3, the GFF file of W. acranulata.File_S4_Wlae.gff.zip: Supplementary File S4, the GFF file of W. laevis.File_S5_Wacr.anti.fas: Supplementary File S5, coding sequences of all antithrombotic genes of W. acranulata.File_S6_Wlae.anti.fas: Supplementary File S6, coding sequences of all antithrombotic genes of W. laevis.水蛭以吸血习性广为人知，且因可分泌多种抗血栓物质而被广泛应用于医药领域。然而，部分巨蛭属（Whitmania）物种并不具有吸血食性，其药用价值尚存争议。本研究报道了光润巨蛭（Whitmania acranulata）与平滑巨蛭（Whitmania laevis）的两个近乎完整的高质量基因组。研究团队同时鉴定了多个参与抗凝、抗血小板聚集、纤维蛋白溶解及药物扩散的抗血栓基因家族。本研究结果将极大推动水蛭衍生物在血栓性疾病医药领域的进化研究与应用。 File_S1_Wacr.genome.zip：补充文件S1，光润巨蛭（W. acranulata）的基因组组装文件。 File_S2_Wlae.genome.zip：补充文件S2，平滑巨蛭（W. laevis）的基因组组装文件。 File_S3_Wacr.gff.zip：补充文件S3，光润巨蛭（W. acranulata）的GFF注释文件。 File_S4_Wlae.gff.zip：补充文件S4，平滑巨蛭（W. laevis）的GFF注释文件。 File_S5_Wacr.anti.fas：补充文件S5，光润巨蛭（W. acranulata）所有抗血栓基因的编码序列。 File_S6_Wlae.anti.fas：补充文件S6，平滑巨蛭（W. laevis）所有抗血栓基因的编码序列。