Effect of global translation initiation inhibition associated to glucose starvation on cytoplasmic lncRNAs levels

To analyze the effect of global translation initiation inhibition associated to glucose deprivation on cytoplasmic lncRNAs levels, we performed RNA-Seq using WT, xrn1-delta and upf1-delta cells grown in glucose-containing complete synthetic medium (CSM) and then shifted for 16 min in glycerol- and ethanol-containing medium. In parallel, control cells were maintained for the same time in glucose-containing CSM. For the WT strains, we also included a treatment of 15 min with CHX (100 μg/ml final concentration) or an equal volume of DMSO (Mock).为探究葡萄糖剥夺相关的全局翻译起始抑制对细胞质长链非编码RNA（lncRNAs）水平的影响，我们针对在含葡萄糖的完全合成培养基（CSM）中培养、随后转移至含甘油与乙醇的培养基中孵育16分钟的野生型（WT）、xrn1Δ与upf1Δ细胞开展了RNA测序（RNA-Seq）实验。与此同时，对照组细胞在含葡萄糖的CSM中维持相同培养时长。针对野生型菌株，我们还设置了终浓度为100 μg/ml的环己酰亚胺（CHX）处理15分钟的实验组，以及加入等体积二甲基亚砜（DMSO）的空白对照（Mock）组。