Dataset for: Characterization of conserved circular RNA in polyploid and its ancestors, Gossypium spp.

Circular RNA (circRNA) is a regulatory class of long, non-coding RNA found in both plant and animal kingdoms. The profile and characterization of circRNA in cotton species remains to be explored. Here, using 24 rRNA-depleted RNA-seq libraries of putative diploid progenitors of Gossypium spp., G. arboreum and G. raimondii, their interspecies hybrid (F1) and allotetraploid G. hirsutum, 1,041, 1,478, 1,311 and 499 circRNAs were identified in each cotton species respectively. A prevalence of 23 of exon-circRNAs are non-canonical GT/AG signals, and only ~10% of exon-circRNA are associated with reverse complementary intronic sequences. This result implies that plants employ a method of circRNA splicing distinct from that of animals. In addition, 432 circRNAs are stably expressed in multiple cotton species.

环形RNA（circRNA）是一类广泛存在于动植物界的长链非编码调控RNA。目前棉属（Gossypium）物种中环形RNA的表达谱与特征解析仍有待深入探索。本研究利用棉属推定二倍体祖先种亚洲棉（G. arboreum）、雷蒙德氏棉（G. raimondii）、二者的种间杂交F1代以及异源四倍体陆地棉（G. hirsutum）的24个核糖体RNA去除型RNA-seq文库，分别在四个棉种中鉴定得到1041、1478、1311和499个环形RNA。研究发现，23%的外显子环形RNA携带非经典GT/AG剪接信号，且仅约10%的外显子环形RNA与反向互补内含子序列相关联。该结果表明植物所采用的环形RNA剪接机制与动物存在显著差异。此外，共有432个环形RNA在多个棉属物种中稳定表达。