Rodrigues et al. The genetic basis and adult reproductive consequences of developmental thermal plasticity

<b>Summary:</b> This data was used to assess how sublethal heat stress during development impacted subsequent multiple reproductive traits in both sexes and their plasticity and to identify candidate genes of reproductive traits sensitive to thermal stress. Using a subset of isogenic lines of the Drosophila Genetic Reference Panel (DGRP) reared at standardized densities in three temperatures (25°C, 27°C, 29°C), we quantified phenotypic variation in mating propensity, fertility, productivity and sex-ratio and obtained their respective thermal reaction norms when applicable. <br> <b>Files available:</b> We provide 2 .txt files (MALE_phenotypicdata; FEMALE_phenotypicdata) and a .xls file (TableS4_genomic_analyses_results_productivity).<br> <b>MALE_phenotypicdata: </b> This file contains the raw data obtained for male DGRP under one of three constant temperatures. Table headers are explained below: <i>Block –</i> week in which the data were collected (1 or 2). <i>LineDGRP -</i> line identity. <i>Line_Status</i> - classification based on the male reproductive performance across three temperatures tested in Zwoinska et al. (2020)*. (High, lines whose fertility did not decline as temperature increased; Low, lines whose fertility declined substantially as temperature increased). <i>Temperature</i> - mean developmental temperature experienced (25°C, 27°C or 29°C). <i>Vial</i> – vial where each pair was kept. <i>Mating</i> – mating propensity (1, if mating observed; 0, if no mating observed) <i>Larvae</i> – fertility (1, if larvae present; 0, if no larvae present) <i>Productivity (1,2 and 3)</i> – number of adult offspring counted on the first, second and third day of offspring counting. <i>Daughters (1, 2 and 3)</i> – number of adult females counted on the first, second and third day of offspring counting. <i>Sons (1, 2 and 3)</i> – number of adult males counted on the first, second and third day of offspring counting. <i>daughtersTotal </i>– Total number of adult females counted. <i>sonsTotal</i>– Total number of adult males counted.<i></i> <i>productivityTotal</i>– Total number of adult offspring counted.<i></i><br> <i> </i> <b>FEMALE_phenotypicdata: </b> This file contains the raw data obtained for female DGRP under one of three constant temperatures. Table headers are explained below: <i>LineDGRP -</i> line identity. <i>Line_Status</i> - classification based on the male reproductive performance across three temperatures tested in Zwoinska et al. (2020)*. (High, lines whose fertility did not decline as temperature increased; Low, lines whose fertility declined substantially as temperature increased). <i>Temperature</i> - mean developmental temperature experienced (25°C, 27°C or 29°C). <i>Vial</i> – vial where each pair was kept. <i>Larvae</i> – fertility (1, if larvae present; 0, if no larvae present) <i>Productivity (1,2 and 3)</i> – number of adult offspring counted on the first, second and third day of offspring counting. <i>Daughters (1, 2 and 3)</i> – number of adult females counted on the first, second and third day of offspring counting. <i>Sons (1, 2 and 3)</i> – number of adult males counted on the first, second and third day of offspring counting. <i>daughtersTotal </i>– Total number of adult females counted. <i>sonsTotal</i>– Total number of adult males counted.<i></i> <i>productivityTotal</i>– Total number of adult offspring counted.<i></i><br><br><b>TableS4_genomic_analyses_results_productivity:</b>This table contains several individual sheets, the legends for these sheets are given on the first sheet of the excel and on the files called "Figshare_information_file".<br><br> *Zwoinska, M. K., Rodrigues, L. R., Slate, J., &amp; Snook, R. R. (2020). Phenotypic responses to and genetic architecture of sterility in response to sub-lethal temperature during development. Frontiers in Genetics, 11, 573. https://doi.org/10.3389/fgene.2020.00573

**数据摘要：** 本数据集用于探究发育过程中的亚致死热胁迫如何影响雌雄两性后续的多项生殖性状及其可塑性，并筛选出对热胁迫敏感的生殖性状候选基因。本研究使用在三种温度（25℃、27℃、29℃）下以标准化饲养密度培育的果蝇遗传参考面板（Drosophila Genetic Reference Panel，DGRP）的等基因系子集，量化了交配倾向、繁殖力、生产力以及性比的表型变异，并在适用条件下获取了各性状对应的热反应规范。

**可用文件：** 本次提供2个.txt格式文件（MALE_phenotypicdata、FEMALE_phenotypicdata）以及1个.xls格式文件（TableS4_genomic_analyses_results_productivity）。

**MALE_phenotypicdata：** 该文件包含在三种恒温之一下饲养的雄性DGRP的原始数据，表格表头说明如下：
* **Block –** 数据收集周次（1或2）。
* **LineDGRP –** 品系编号。
* **Line_Status –** 基于Zwoinska等（2020）研究中三种温度下雄性生殖表现划分的品系分类：（High：随温度升高繁殖力未下降的品系；Low：随温度升高繁殖力显著下降的品系）。
* **Temperature –** 个体发育所处的平均温度（25℃、27℃或29℃）。
* **Vial –** 对应供试果蝇对的饲养管编号。
* **Mating –** 交配倾向（观察到交配记为1，未观察到交配记为0）。
* **Larvae –** 繁殖力（存在幼虫记为1，无幼虫记为0）。
* **Productivity (1、2和3) –** 子代计数第1、2、3天统计的成虫子代总数。
* **Daughters (1、2和3) –** 子代计数第1、2、3天统计的成虫雌性个体数。
* **Sons (1、2和3) –** 子代计数第1、2、3天统计的成虫雄性个体数。
* **daughtersTotal –** 统计的成虫雌性个体总数。
* **sonsTotal –** 统计的成虫雄性个体总数。
* **productivityTotal –** 统计的成虫子代总数。

**FEMALE_phenotypicdata：** 该文件包含在三种恒温之一下饲养的雌性DGRP的原始数据，表格表头说明如下：
* **LineDGRP –** 品系编号。
* **Line_Status –** 基于Zwoinska等（2020）研究中三种温度下雄性生殖表现划分的品系分类：（High：随温度升高繁殖力未下降的品系；Low：随温度升高繁殖力显著下降的品系）。
* **Temperature –** 个体发育所处的平均温度（25℃、27℃或29℃）。
* **Vial –** 对应供试果蝇对的饲养管编号。
* **Larvae –** 繁殖力（存在幼虫记为1，无幼虫记为0）。
* **Productivity (1、2和3) –** 子代计数第1、2、3天统计的成虫子代总数。
* **Daughters (1、2和3) –** 子代计数第1、2、3天统计的成虫雌性个体数。
* **Sons (1、2和3) –** 子代计数第1、2、3天统计的成虫雄性个体数。
* **daughtersTotal –** 统计的成虫雌性个体总数。
* **sonsTotal –** 统计的成虫雄性个体总数。
* **productivityTotal –** 统计的成虫子代总数。

**TableS4_genomic_analyses_results_productivity：** 该表格包含多个独立工作表，各工作表的图例说明位于该Excel文件的第一张工作表以及名为"Figshare_information_file"的文件中。

*Zwoinska, M. K., Rodrigues, L. R., Slate, J., & Snook, R. R. (2020). 发育过程中亚致死温度胁迫导致的不育表型响应及其遗传基础. 遗传学前沿, 11, 573. https://doi.org/10.3389/fgene.2020.00573