Development of UPLC-MS/MS method for determining hainanmycin in foods of animal origin

Hainanmycin is a polyether antibiotic. Toxicological studies have shown the adverse effects of hainanmycin on animals and humans. At present, no study is available on the detection of hainanmycin in edible tissues of animals. Hence, a fast and accurate detection method for hainanmycin is essential. This study aimed to develop a new analytical method based on ultra-high-performance liquid chromatography-tandem mass spectrometry to detect hainanmycin in 10 matrices, including milk, eggs, fat, kidney, muscles and livers of chicken, beef and sheep. The limit of detection and the limit of quantitation of the 10 matrices were 0.1–0.4 μg/kg and 0.25–1 μg/kg, respectively, and were far below the maximum residue limits of other polyether anticoccidial drugs (1–150 μg/kg). The recoveries of hainanmycin ranged from 79% to 105%, and the relative standard deviation ranged from 2.8% to 12.0%. The research results prove that the proposed method is operational and simple in detecting hainanmycin, and has high precision and accuracy in a variety of matrices.

海南霉素（Hainanmycin）属于聚醚类抗生素（polyether antibiotic）。毒理学研究已证实其对动物及人体存在不良影响。目前尚无针对动物可食用组织中海南霉素检测的相关研究，因此开发快速准确的海南霉素检测方法具有重要意义。本研究旨在建立一种基于超高效液相色谱-串联质谱法（ultra-high-performance liquid chromatography-tandem mass spectrometry）的新型分析方法，用于检测牛奶、鸡蛋、鸡、牛、羊的脂肪、肾脏、肌肉及肝脏共10种基质中的海南霉素。该方法在10种基质中的检测限与定量限分别为0.1~0.4 μg/kg和0.25~1 μg/kg，远低于其他聚醚类抗球虫药的最大残留限量（1~150 μg/kg）。海南霉素的回收率范围为79%~105%，相对标准偏差范围为2.8%~12.0%。研究结果证明，本方法用于检测海南霉素操作简便、实用性强，且在多种基质中均具备较高的精密度与准确度。