Conformational transitions and activation of the adhesion receptor CD97

CD97, also known as ADGRE5, is the first cloned human aGPCR that belongs to the epidermal growth factor (EGF) seven-transmembrane family of aGPCRs (now called ADGRE family). We reported the cryo-electron microscopy structures of human CD97 in its inactive apo and G13-bound fully active states. Here, we present SDS-PAGE analysis results for the purified inactive CD97 and CD97-G13 complex.

CD97，亦称ADGRE5，是首个被克隆的人源黏附类G蛋白偶联受体（aGPCR，adhesion G protein-coupled receptor），隶属于aGPCR的表皮生长因子（EGF）七跨膜家族（现命名为ADGRE家族）。我们此前解析了人源CD97分别处于非激活无配体（apo）态与结合G13的完全激活态的冷冻电子显微镜结构。本文中，我们呈现了纯化的非激活态CD97以及CD97-G13复合物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析结果。