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Additional file 13 of Dynamic nanopore long-read sequencing analysis of HIV-1 splicing events during the early steps of infection

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DataCite Commons2020-08-25 更新2024-07-28 收录
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Additional file 13: Table S6. ONT read mapping statistics of T cell sample infected with HIV-1 between 12 h and 24 h. Primary CD4+ T cells from donor 4 were infected with VSV-G pseudotyped NL4-3 virus, RNA was extracted at 12, 14, 16, 20, 24 hpi, cDNA libraries were prepared and sequenced using ONT device. ONT sequencing reads were mapped to both the human and HIV NL4-3 genomes, using Minimap2.

补充文件13:表S6。感染HIV-1的T细胞样本在12 h至24 h期间的牛津纳米孔(ONT)读段比对统计数据。来自供体4的原代CD4+ T细胞被VSV-G假型化的NL4-3病毒感染,分别于感染后12、14、16、20、24小时(hpi)提取RNA,构建cDNA文库并使用ONT测序设备进行测序。使用Minimap2工具将ONT测序读段比对至人类基因组与HIV NL4-3基因组。
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figshare
创建时间:
2020-08-18
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