Genome wide mapping of sni445-HTP binding sites in yeast by CRAC

We report the application of the Cross-Linking and cDNA (CRAC) technique to identify binding sites of sni445, ribosome assembly factor, on RNAs in vivo. Once sequenced, RNAs associated have been aligned to the yeast genome and enrichment of specific RNAs has been studied in more details. Specifically, apparition of Mutations/deletions in the reads is center of interest since they mostly correspond to precise site of cross-link between bait protein and target RNAs. Here sni445-HTP is mostly bound to two snoRNAs, SNR4 and SNR45. Overall design: We analyse the sequence enrichment of RNAs associated to sni445-HTP compared to a mock (un-tagged) control after protein/RNA cross-link in vivo.