Supplement with Cystoseira canariensis promotes an increase of resistance to the maximum load in the grastrocnemius muscle of female rats

Was evaluated the maximum load supported by the grastrocnemius muscle of female rats after the ministering of Cystoseira canariensis, either associated or not to swimming. Twenty-eight young Wistar female rats were used, divided into 4 groups: control (C, n=7); supplement (S, n=7); supplement and swimming (SSw, n=7); swimming (Sw, n=7). Each one pertaining to the groups S and SSw received 20 mg of myostatin inhibitor per day. The swimming consisted in an aerobe protocol, three times in a week, during eight weeks. The right grastrocnemius muscle of each animal was removed and a tension test was performed in an Emic testing machine. The results (Mean±SEM) were evaluated through ANOVA and Tukey test (p<0.05). A significant difference for maximum load (in N) was verified among the groups C (35.41±1.06) and S (39.98±1.15); Sw (27.94±2.19) and S (39.98±1.15); Sw (27.94±2.19) and SSw (37.78±1.28). In relation to the stretching at the maximum limit (in x10-3m) at the maximum load, the group SSw obtained a value (20.68±1.19) significantly greater than the groups C (17.15±1.11), S and Sw (16.11±1.60). There was a significant difference for body weight gain among the groups treated with supplement and supplement associated to the swimming, with smaller values for this last. The myostatin inhibitor either, associated or not to the swimming, promotes an increase of resistance to the maximum load in the tension test in grastrocnemius muscle of young female rats.

本研究评估了给予加那利囊叶藻（Cystoseira canariensis，作为膳食补剂）并联合或不联合游泳运动时，雌性大鼠腓肠肌（gastrocnemius）的最大载荷承受能力。本实验共纳入28只年轻雌性Wistar大鼠（Wistar），随机分为4组：空白对照组（C，n=7）、囊叶藻补剂组（S，n=7）、囊叶藻补剂联合游泳组（SSw，n=7）以及单纯游泳组（Sw，n=7）。其中囊叶藻补剂组与囊叶藻补剂联合游泳组的大鼠每日给予20 mg肌抑素抑制剂（myostatin inhibitor）。游泳运动采用有氧训练方案，每周进行3次，持续8周。实验结束后，摘取每只大鼠的右侧腓肠肌，使用Emic试验机开展张力测试。实验结果以均值±标准误（Mean±SEM）表示，通过单因素方差分析（ANOVA）与Tukey检验进行统计学分析，检验水准设定为p<0.05。结果显示：最大载荷（单位：N）方面，空白对照组（C，35.41±1.06）与囊叶藻补剂组（S，39.98±1.15）、单纯游泳组（Sw，27.94±2.19）与囊叶藻补剂组（S）、单纯游泳组（Sw）与囊叶藻补剂联合游泳组（SSw，37.78±1.28）之间均存在显著统计学差异；最大载荷下的极限拉伸量（单位：×10⁻³ m）方面，囊叶藻补剂联合游泳组（SSw，20.68±1.19）的数值显著高于空白对照组（C，17.15±1.11）以及囊叶藻补剂组（S）与单纯游泳组（Sw，16.11±1.60）；体重增重方面，接受囊叶藻补剂治疗与囊叶藻补剂联合游泳治疗的两组间存在显著差异，后者的体重增重更低。本研究表明，肌抑素抑制剂无论联合游泳运动与否，均可提升年轻雌性大鼠腓肠肌在张力测试中的最大载荷抵抗能力。