RNA-seq of FACS-enriched mammary subpopulations (basal, luminal progenitor, mature luminal) from WT and ID4 knockout mice

The goal of this study was to determine the genes regulated by ID4 in mammary epithelial subpopulations. Basal, luminal progenitor, and mature luminal mammary epithelial subpopulations were FACS-sorted from adult mice that were either WT or ID4 KO in quadruplicate. RNA-seq and differential gene expression analysis was performed. Overall design: Mammary glands were pooled from 4-8 WT or ID4 KO female adult (10-12 week) mice at estrus and digested into a single cell suspension. Live lineage negative CD24+CD29hiCD61+ basal, CD24+CD29loCD61+ luminal progenitor and CD24+CD29loCD61- mature luminal cells were isolated by FACS and RNA was extracted for RNA-seq. 4 biological replicates were performed.