Role of RNA binding protein PgRBP1 in alteration of protein profile of Porphyromonas gingivalis

The oral periodontal pathogen Porphyromonas gingivalis must adapt to ever changing environment to survive and cause disease. So far most of the efforts concerning the regulatory mechanisms employed by the bacterium centered on DNA-binding regulators. Here we focused on a RNA-binding protein that is indispensable for the bacterium to survive with host cells. We have determined the proteome affected by deletion of the protein, designated here PgRBP1. Three strains were used: WT, PgRBP1-deficient strain designated V3139 and a complemented strain V3236. In total, 1,393 proteins were identified. 592 proteins in the comparison of P. gingivalis 3139 vs. WT, 228 proteins in the comparison of P. gingivalis 3236 vs. WT, and 276 proteins in the comparison of P. gingivalis 3139 vs. 3236 showed differential level with statistical significance