Protein alignments of human TRIM28 with the putative orthologs from Xenopus and lobe-finned fish species.

Sequences are designated according to their database accession numbers (NCBI Refseq or ENSEMBL) where available and a species prefix (hsap = Homo sapiens; xlae = Xenopus laevis; xtro = Xenopus tropicalis; pann = Protopterus annectens (lungfish); lcha = Latimeria chalumnae (coelacanth)). The lungfish sequences do not bear official identifiers yet and are named arbitrarily here. They were obtained as described in Materials and Methods (Bioinformatics section). The original transcript sequences are provided in Table S2. The consensus sequence under the alignments is based on the occurence of the shown amino acids in at least 60% of the molecules. Reciprocal BLASTp of the frog and fish sequences against human sequence databases resulted in human TRIM28 as the best hit and thus supported the ortholog assignment (data not shown). Boxes around and labels under the sequence alignments delineate the domain organization based on human TRIM28 [80].