The ARID1A tumor suppressor controls global transcription via pausing of RNA Polymerase II. The ARID1A tumor suppressor controls global transcription via pausing of RNA Polymerase II

We investigated the genomic consequences of the depletion of the SWI/SNF subumit ARID1A, in an ovarian cancer derived model. We produced genome wide data for nascent RNA (GRO-seq) and steady state RNA (total RNA-seq). Further, we used ChIP-seq to examine genome-wide distribution of ARID1A, as well as the changes in occupancy of RNAPII, H3K4me3 and H3K36me3. Finally we used ATAC-seq to investigate chromatin accessibility. Overall design: Analysis of global transcriptional changes associated to the depletion of ARID1A.