Table 2 in Taxonomic separation by isozyme electrophoresis of two closely related species of Cicada L. (Hemiptera: Cicadoidea) in Portugal

Table 2. Activity of buffer/enzyme combinations screened in preliminary electrophoretic work with Cicada orni and C. barbara. Buffer²E.C. no.Enzyme systemTris-Citrate pH 6.0Tris-Citrate pH 6.7Tris-Citrate pH 8.0LiOH pH 8.32.6.1.1Aspartate aminotransferase (AAT)021━3.5.4.4Adenosine deaminase (ADA)━11━2.7.4.3Adenylate kinase (AK)━22━1.1.1.1Alcohol dehydrogenase (ADH)━1101.2.3.1Aldehyde oxidase (AO)━1122.7.3.2Creatine kinase (CK)━22━3.1.1.1Esterase (EST)01102.7.1.4Frutokinase (FK)000━1.1.1.49Glucose-6-phosphate dehydrogenase (G6PDH)━00━5.3.1.9Glucose phosphate isomerase (GPI)01121.1.1.47Glucose dehydrogenase (GLC)01001.4.1.2Glutamate dehydrogenase (GLD)01101.1.1.8Glycerol-3-phosphate dehydrogenase (α -GPD)━2222.7.1.1Hexokinase (HK)━220General protein (GP)━1111.1.1.42Isocitrate dehydrogenase (IDHP)━22━1.1.1.27Lactate dehydrogenase (LDH)00001.1.1.37Malate dehydrogenase (MDH)━22━1.1.1.40Malic enzyme (ME)02215.3.1.8Mannosephosphate isomerase (MPI)━221Peptidase (PEP)━━━21.1.1.43Phosphogluconate dehydrogenase (PGDH)━1202.7.5.1Phosphoglucomutase (PGM)━2211.1.1.14Sorbitol dehydrogenase (SDH)━1101.15.1.1Superoxide dismutase (SOD)━1111.1.1.204Xanthine dehydrogenase (XDH)━000 ━ Indicates buffer/enzyme combination not screened; 0 indicates no activity; 1 indicates low activity or resolution insu ffi cient to allow clear assessment; 2 indicates good activity with scorable locus. ²Buffers described in Pasteur et al. (1987).