A R2R3-MYB transcriptional activator EbMYBP1 regulates flavonoid biosynthesis

Erigeron breviscapus is rich in flavonoids, flavonols and their glycosides that are beneficial to human health. While we know that R2R3-MYB transcription factors (TFs) play a vital role in the flavonoid pathway, the transcriptional regulation of flavonoid biosynthesis in E.breviscapus remains poorly understood. In this study, EbMYBP1, a R2R3-MYB TF, was identified as a regulator involved in the regulation of flavonoid accumulation. Transcriptome and metabolome exploration revealed that a large group of genes in the phenylalanine pathway related to flavonoid biosynthesis were significantly changed, accompanied by significantly increased concentrations of the flavonoid in EbMYBP1-OE transgenic tobacco compared to the wild-type (WT).