Pakistan Cotton Biophysical Survey, 2013

<p>To study the efficiency of genetically modified cotton markets in Pakistan, a series of household surveys were designed and implemented in 2013. Aside from the four rounds of household surveys, the research team also designed a biophysical survey. The purpose of the biophysical survey was to measure the presence and expression level of Cry protein in farmers’ fields. The plot selected to collect tissue samples to be tested was the “main” cotton plot identified in the household surveys implemented in 2013-14. </p>
<p>The collection, tests, and analysis of the cotton tissue (leaves and bolls) was led by the University of Agriculture, Faisalabad (UAF), and the National Institute for Genomics and Advanced Biotechnology (NIGAB), Islamabad, in collaboration with International Food Policy Research Institute (IFPRI), and Innovative Development Studies. Two different tests were carried out. The first one was the strip test that test for the presence or absence of the Cry1Ac protein. The second was the enzyme-linked immunosorbent assay (ELISA) that quantifies the expression level of this protein. Two rounds of tests were conducted, the first at approximately 70 days after sowing (DAS) and the second at approximately 120 DAS. The resultant dataset combines information from 593 sampled households with corresponding plant tissue diagnostics from 70 DAS, as well as information from 589 sampled households with corresponding diagnostics from 120 DAS.
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为探究巴基斯坦转基因棉花市场的运行效率，研究团队于2013年设计并实施了一系列农户调查。除四轮农户调查外，该团队还同步设计了一项生物物理调查，旨在测定农户田间Cry蛋白（Cry protein）的存在情况与表达水平。本次采集待测组织样本的棉田，为2013-2014年农户调查中确定的“主要”棉田。

棉花组织（叶片与棉铃）的采集、检测与分析工作由费萨拉巴德农业大学（University of Agriculture, Faisalabad, UAF）与伊斯兰堡国家基因组与先进生物技术研究所（National Institute for Genomics and Advanced Biotechnology, NIGAB）牵头开展，并与国际粮食政策研究所（International Food Policy Research Institute, IFPRI）及创新发展研究机构（Innovative Development Studies）合作完成。本次研究共开展两类检测：其一为试纸条检测，用于定性判定Cry1Ac蛋白（Cry1Ac protein）的有无；其二为酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA），用于定量测定该蛋白的表达水平。检测共分两轮进行：第一轮于播种后约70天（days after sowing, DAS）开展，第二轮于播种后约120天进行。最终生成的数据集整合了593户抽样农户的相关信息，及其对应播种后70天的植物组织检测结果，同时纳入了589户抽样农户的相关信息，及其对应播种后120天的植物组织检测结果。