Integrated transcriptomic analysis of Trichosporon asahii uncovers the core genes and pathways of fluconazole resistance

We performed a series of fluconazole (FLC) perturbation experiments for two T. asahii strains, a clinical isolate stain CBS2479 (T2) and an environmental isolate strain CBS8904 (T8), to uncover potential genes and pathways involved in FLC resistance. Overall design: We treated both types of T. asahii cells with two different doses of FLC for 5 different time points respectively, and then collected RNAs for gene expression profiling.