Metabolic changes in cancer cells after metformin treatment

Proliferation measured by confluency in Incucyte ZOOM. Viability by CCK8, Alamar Blue, and direct cell counting microscopically (metformin concentration range: 1.5-24 mM). Metabolic analysis usuaing the Seahorse XF96 (metformin exposure for 48 hours, 3 mM). GLUT1 expression measured via flow cytometry. Detailed methods found in published article.

本数据集的检测内容如下：细胞增殖以细胞汇合度为检测指标，通过Incucyte ZOOM活细胞成像系统完成测定；细胞活力通过CCK8法、阿尔玛蓝（Alamar Blue）法以及显微镜下直接细胞计数法检测（二甲双胍处理浓度范围为1.5~24 mM）；代谢分析采用Seahorse XF96细胞外流量分析仪开展，二甲双胍暴露处理时长为48小时、处理浓度为3 mM；葡萄糖转运蛋白1（GLUT1）的表达通过流式细胞术检测。详细实验方法参见已发表的研究论文。