Resistance of tomato plant genotypes with high foliar allelochemical contents to the leafminer Liriomyza trifolii

ABSTRACT: The objective of this study was to assess the resistance of tomato genotypes with high foliar levels of allelochemicals to the leafminer Liriomyza trifolii. Eight tomato genotypes with contrasting levels of three different alellochemicals were tested for leafminer resistance: the TOM-687 and TOM-688 lines, which are both rich in acylsugar; ZGB-703 and ZGB-704, which are rich in zingiberene; BPX-365G-899-07-04-02 and BPX-367E-238-02, which are rich in 2-tridecanone; and 2 standard commercial hybrids (Deborah Max and Bravo F1) with low-allelochemical content. Thirty days after being transplanted, nine potted plants of each of the treatments were transferred to a phytotron chamber, into which 1600 unsexed leafminer adults from a mass rearing were released. The number of mines and the percentage of leaves and leaflets attacked (= with the presence of punctures) were evaluated. Six days later, the number of pupae was recorded. The genotypes with high levels of acylsugar were associated with higher levels of resistance to leaf miner L. trifolii, through antixenosis and antibiosis mechanisms. At least one of the 2-tridecanone-rich lines (BPX -365G-899-07-04-02) showed some level of resistance via an antibiosis mechanism.

摘要：本研究旨在评估叶片化感物质（allelochemicals）含量较高的番茄基因型对美洲斑潜蝇（Liriomyza trifolii）的抗性。本试验共选取8种番茄基因型，其3种不同化感物质的含量梯度差异显著：分别为富含酰基糖（acylsugar）的TOM-687与TOM-688品系、富含姜烯（zingiberene）的ZGB-703与ZGB-704品系、富含2-十三烷酮（2-tridecanone）的BPX-365G-899-07-04-02与BPX-367E-238-02品系，以及2种化感物质含量较低的商业标准杂交品种Deborah Max与Bravo F1。移栽30天后，将每个处理组的9盆盆栽植株转移至人工气候室（phytotron chamber），接入1600头来自批量饲养种群的未交配斑潜蝇成虫。试验评估指标包括虫道数量、受侵害叶片及小叶的百分比（即存在刺吸痕迹的组织）；接虫6天后记录蛹的数量。结果显示，高酰基糖含量的番茄基因型通过驱避性（antixenosis）与抗生性（antibiosis）两种机制，对美洲斑潜蝇表现出更强的抗性；而富含2-十三烷酮的品系中至少有一个（BPX-365G-899-07-04-02）可通过抗生性机制展现出一定水平的抗性。