Induction of resistance to Colletotrichum truncatum in lima bean

ABSTRACT: Anthracnose (Colletotrichum truncatum) is one of the major diseases of the lima bean culture, found in production fields, causing decrease in productivity. The objective of this study was to evaluate the effect of abiotic and biotic inducers applied in lima bean plants to reduce anthracnose severity. Lima bean accessions were used and treated with: the abiotic inducers calcium silicate (Agrosilício Plus) and silicate clay (Rocksil), using the 3 g dose.L-1; the biotic inducer citric biomass extract (Ecolife), at the dose of 3 mL.L-1; and distilled water as control. To evaluate the resistance induction, the results of severity and degree of resistance of fava bean at 7, 11, 15, 19 and 23 days after inoculation were considered according to the scale of grades and classes of reactions. The design was a randomized block in a factorial arrangement 4 × 15 (treatments × hits) with four blocks. Resistance inducers Agrosilicon Plus, Ecolife and Rocksil have potential to be used in the management of anthracnose in fava bean. The studied accesses showed degree of resistance, being able to be explored in relation to obtaining anthracnose resistant cultivars in future breeding programs of this crop.

摘要：由截断刺盘孢（Colletotrichum truncatum）引发的炭疽病是利马豆（lima bean）栽培中的主要病害之一，广泛发生于生产田块，可导致产量下降。本研究旨在评估施用于利马豆植株的非生物与生物诱导剂对减轻炭疽病病情的效果。试验采用利马豆种质，分别施加以下处理：非生物诱导剂硅酸钙（Agrosilício Plus）与硅酸盐黏土（Rocksil），施用剂量为3 g·L⁻¹；生物诱导剂柠檬酸生物质提取物（Ecolife），剂量为3 mL·L⁻¹；以蒸馏水作为空白对照。为评估抗病诱导效果，本研究根据病害分级与反应类别标准，统计了接种后7、11、15、19及23天时蚕豆（fava bean）的病情严重度与抗病等级。试验设计为随机区组设计，采用4×15因子排列（处理×重复），共设置4个区组。研究结果表明，抗病诱导剂Agrosilício Plus、Ecolife与Rocksil具备用于利马豆炭疽病防控的应用潜力。本研究所用的种质材料表现出不同程度的抗病性，可在该作物未来的抗病育种项目中用于培育抗炭疽病品种。