Downregulation of MED12 via siRNA in SPHEROIDS derived from prostate cancer cell lines (3' tag DGE)

The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance. Overall design: To investigate the relevance of MED12 in spheroids derived from enzalutamide sensitive and resistant prostate cancer cells, we performed siRNA mediated knockdown, generated spheroids and analyzed the response using high throughput 3' tag differential gene expression analysis.