five

12h pathway stimulation / inhibition experiment in mouse ES cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17136
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This experiment records the transcriptional responses of mES cells (line OG2) to FGF/ERK stimulation in the presence of LIF, to LIF/STAT3 inhibition in the presence of an FGF/ERK inhibitor, and to combined FGF/ERK stimulation / LIF/STAT3 inhibition. ES cells were grown feeder-free on gelatin in KSR medium containing an FGF/ERK inhibitor and LIF and were then treated for 12h as indicated for the individual samples.
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2018-06-14
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