Laboratory results containing transcripts of four cultivated marine bacteria from 2014-2015 (EAGER Respiration project)

<p>The RNA-seq data have been deposited with&nbsp;NCBI under GenBank Accession Number <a href=\"http://www.ncbi.nlm.nih.gov/bioproject/PRJNA276979/\" target=\"_blank\">PRJNA276979</a>.</p>

<p>Bacteria respond to stimuli in the environment using transcriptional control, but this may not be the case for most marine bacteria having small, streamlined genomes. Candidatus Pelagibacter ubique, a cultivated representative of the SAR11 clade, which is the most abundant clade in the oceans&nbsp;4, has a small, streamlined genome and possesses an unusually small number of transcriptional regulators. This observation leads to the hypothesis that transcriptional control is low in Pelagibacter and limits its response to environmental conditions. However, the extent of transcriptional control in Pelagibacter is unknown. Overall design: Bacteria were grown in batch culture and sampled twice during the initial, rapid phase of exponential growth and twice during the phase of slower growth that followed.&nbsp; Transcripts deposited here were assessed using RNA-seq.</p>

本研究的RNA测序（RNA-seq）数据已提交至美国国家生物技术信息中心（NCBI），登录号为PRJNA276979，相关信息可通过<a href="http://www.ncbi.nlm.nih.gov/bioproject/PRJNA276979/" target="_blank">此链接</a>查询。

细菌可通过转录调控响应环境刺激，但对于多数基因组小巧精简的海洋细菌而言，这一调控机制可能并不适用。海洋中丰度最高的细菌类群为SAR11演化支（SAR11 clade）[4]，候选Pelagibacter ubique（Candidatus Pelagibacter ubique）是该演化支的可培养代表菌株，其基因组小巧精简，转录调控因子的数量异乎寻常地少。基于这一现象，学界提出如下假说：Pelagibacter的转录调控水平较低，这限制了其对环境条件的响应能力。然而，目前关于Pelagibacter转录调控的范围仍不明确。本实验整体设计如下：将细菌于分批培养（batch culture）中培养，在指数生长初期的快速增殖阶段采集两次样本，在后续的缓慢生长阶段再采集两次样本。本数据集所提交的转录本通过RNA-seq技术完成了测序评估。