Additional file 1: of Key factors identified by proteomic analysis in maize (Zea mays L.) seedlings’ response to long-term exposure to different phosphate levels

Figure S1. Phenotypic responses of QXN233 genotype to LP or HP condition. QXN233 grown under the different Pi-treated conditions for 10 days (a) via a vermiculite assay or for 20 days (b) via a hydroponic assay. Bar = 5 cm, Bar = 2 cm. Figure S2. Phenotypic responses of QXN233 genotype to LP or HP condition. QXN233 grown under the different Pi-treated conditions for 25 days (a) via a vermiculite assay. Bar = 10 cm. Table S1. Primers used in qRT-PCR. Table S2. Quantitative analyses of plant height and the width and length of the longest leaf in QXN233 after 30 days under 0 mM Pi or 3 mM Pi via vermiculite assay. Values represent means ± SEM of three replicates. Asterisks indicate a significant difference between the Pi-treated and control groups (LSD test, P < 0.05). Table S3. DEPs of QXN233 identified under low or high Pi (LP or HP) compared with the normal condition via the proteomic analysis (Ratio |0 Pi or 3 Pi/Control| > 1.2 and P < 0.05). The red and green markers presented the upregulated and downregulated values of DEPs, respectively. Table S4. Dataset.xlsx. (ZIP 4300 kb)

补充图S1。QXN233基因型对低磷（LP）或高磷（HP）条件的表型响应。QXN233经不同Pi处理后，分别通过蛭石培养测定培养10天（a），或通过水培测定培养20天（b）。标尺 = 5 cm，第二标尺 = 2 cm。补充图S2。QXN233基因型对低磷（LP）或高磷（HP）条件的表型响应。QXN233经不同Pi处理后通过蛭石培养测定培养25天（a）。标尺 = 10 cm。补充表S1。实时定量反转录聚合酶链式反应（quantitative real-time reverse transcription PCR, qRT-PCR）所用引物。补充表S2。通过蛭石培养测定，在0 mM Pi或3 mM Pi条件下培养30天后，QXN233的株高、最长叶的叶宽和叶长的定量分析结果。数值以三次生物学重复的平均值±标准误（standard error of the mean, SEM）表示。星号表示Pi处理组与对照组之间存在显著差异（最小显著差检验（least significant difference test, LSD），P < 0.05）。补充表S3。通过蛋白质组学分析，相较于正常培养条件，在低磷或高磷（LP或HP）条件下鉴定得到的QXN233差异表达蛋白（differentially expressed proteins, DEPs），筛选标准为|0 mM Pi组或3 mM Pi组/对照组|的比值 > 1.2且P < 0.05。其中红色和绿色标记分别代表差异表达蛋白的上调和下调表达量。补充表S4。Dataset.xlsx文件（压缩包大小4300 kb）。