Chronic stress associated to contraceptives use on the progression of ligature-induced periodontitis in rats

Abstract Introduction Contraceptives are widely used by women in much of the world. The stress associated with their use can lead adverse responses in the body. Objective The aim of this paper was to evaluate the effects of chronic stress associated with contraceptive use on the progression of ligature-induced periodontitis in female Lewis rats. Material and method Therefore, forty rats were randomly divided into 5 groups (n = 8): contraceptive and ligature group (CG); stress and ligature group (SG); contraceptive, stress and ligature group (CSG); ligature group (LG); and control group (CtrlG). An injectable contraceptive was administered to the CG and CSG groups at the beginning of the experiment. On the following day, periodontitis was induced in the CG, SG, LG and CSG groups by placing ligatures on the upper-right second molar. On the third day, the SG and the CSG were subjected to a chronic stress model using physical restraint and cold exposure. After 60 days, the animals were euthanized and submitted to histometric measurements. Two parameters were considered: histological attachment loss (CEJ-PL) and bone loss (CEJ-BC). Regarding CEJ-PL/CEJ-BC. Result The results indicated that the CtrlG (CEJ-PL 0.10 ± 0.00; CEJ-BC 0.34 ± 0.02) showed the lowest values (p<0.05). The SG group (CEJ-PL 0.90 ± 0.24; CEJ-BC 1.30 ± 0.28) presented higher values that were significantly different (p<0.05) from the results of the LG. The other groups exhibited similar statistical results (p>0.05). Conclusion The present results do not allow an assessment of the susceptibility of animals to ligature-induced periodontitis.

摘要：
引言：避孕药在全球多数地区的女性群体中应用十分普遍。使用避孕药所伴随的应激反应可引发机体产生不良反应。
目的：本研究旨在评估与避孕药使用相关的慢性应激对雌性Lewis大鼠结扎诱导性牙周炎（ligature-induced periodontitis）进展的影响。
材料与方法：将40只大鼠随机分为5组，每组8只：避孕药+结扎组（CG）、应激+结扎组（SG）、避孕药+应激+结扎组（CSG）、结扎组（LG）及对照组（CtrlG）。实验初始阶段，为CG组与CSG组大鼠注射注射型避孕药。次日，通过在右上第二磨牙处放置结扎线，于CG、SG、LG及CSG组大鼠体内诱导牙周炎模型。造模第3天，对SG组与CSG组采用束缚应激联合冷暴露的方式构建慢性应激模型。造模60天后，对所有大鼠实施安乐死，并进行组织形态计量学检测。本次检测选取两项观测参数：组织学附着丧失（CEJ-PL）与骨丧失（CEJ-BC），分析指标为CEJ-PL与CEJ-BC的比值。
结果：结果显示，对照组（CtrlG）的CEJ-PL为0.10±0.00，CEJ-BC为0.34±0.02，两项指标均为最低水平（p<0.05）。应激+结扎组（SG）的CEJ-PL为0.90±0.24，CEJ-BC为1.30±0.28，其指标水平显著高于结扎组（LG）（p<0.05）。其余各组的统计学结果无显著差异（p>0.05）。
结论：本研究结果尚不足以评估大鼠对结扎诱导性牙周炎的易感性。