Raw Diffraction Images Of Leukotriene B4 Receptor Blt1

Diffraction images of Leukotriene B₄ receptor (BLT1) in complex with antagonist BIIL260, a chemical bearing a benzamidine moiety. BLT1 is one of class-A G-protein coupled receptor (GPCR), and the bound benzamidine moiety mimics a sodium ion-centered water cluster of BLT1 that is considered to exist in many class-A GPCRs. The bound benzamidine moiety stabilizes the inactive state of BLT1, and BIIL260 exhibits the characteristics of an inverse agonist for BLT1. Small-wedge (1296 crystals, 2.5°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. The crystals belonged to space group P22₁2₁ with unit cell parameters a=69.6, b=77.6, c=135.5 Å. Finally, 494 datasets were merged at 3.7 Å resolution in the published result (Hori et al. Nature Chemical Biology, 2018; PDB code: 5X33) using KAMO; see processing note https://github.com/keitaroyam/yamtbx/wiki/Processing-BLT1-data-(5X33) Note The master.h5 files were modified; see https://github.com/keitaroyam/yamtbx/blob/master/doc/eiger-en.md To further reduce total file size, flatfield data of master.h5 were removed and saved in a file in each directory (linked from master.h5 files). Most frames have lipid rings.