Multiplex real-time PCR genotyping algorithm validation with biological samples.

Positive results obtained with the Real-Time and conventional PCR assays for the DTU characterization of biological samples were compared. The number of samples belonging to each DTU group corresponds to the Real-Time PCR algorithm results.aEleven peripheral blood samples and one skin biopsy samplebSixty three urine/feces samples on filter paper and 25 abdomen/tissue samplescForty peripheral blood and 4 heart explant samples; pos, positive results. Mixed infections were characterized as dTcV plus TcVI,e6 TcI plus TcIV, 1 TcI plus TcIII/IV and 2 TcIII plus TcIV,fTcI plus TcII. AI, acute T. cruzi infection; ACD, asymptomatic chronic Chagas disease; SCD, symptomatic chronic Chagas disease; CI, congenitally infected children; and RCD, patients with reactivation in the context of immunosuppression.Multiplex real-time PCR genotyping algorithm validation with biological samples.