MouseLight Neuron AA0370

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions. <br>Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long.<br>Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens. <br>The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.

詹妮莉亚（Janelia）科研团队开展的大规模神经示踪项目，将小鼠大脑中已完成完整示踪的神经元数量提升了10倍。如今研究人员可下载并以三维形式浏览该数据集。<br>在小鼠大脑内部，单个神经元会在左右脑半球间蜿蜒穿行，编织出繁复的分支模式；研究人员此次还发现，神经元甚至可延伸出长达45厘米的纤细纤维。<br>科学家们可通过这一迄今最精细的小鼠大脑连接图谱，以三维形式观察并探索这些蜿蜒的神经示踪结构。该图谱重建了小鼠大脑7000万神经元中超过300个的形态与位置。此前的单个神经元路径示踪研究最多仅能完成数十个神经元的示踪。<br>这些经选择性标记的神经元通过双光子显微镜（two-photon microscopy）的迭代流程完成成像。实验中将大脑切成200微米厚度的脑片，每次对数十个神经元进行标记并成像。每颗成像的大脑可产生约50太字节（terabytes）的数据，其中包含的已标注神经元可通过NeuronBrowser应用程序进行浏览。若研究人员需要完整的原始数据集，可联系詹妮莉亚团队，通过硬件转移的方式获取相关数据。