Dynamics of transcriptomes of hPSCs treated by CHIR99021 for 21 hours
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE142572
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We have evidence to demonstrate that hPSCs enter mesoderm or endoderm lineages dependent on the dose of WNT signaling activation. To understand the underlying mechanism of CHIR99021-induced definitive endoderm (DE) differentiation, we collected hPSCs treated by CHIR99021 for 21 hours and performed RNA sequencing, to investigate the dynamics of transcriptomes in every three hours post CH treatment. 19-9-11 hiPSCs were treated with 6 uM CHIR99021 in RPMI medium and cells were collected for RNA sequencing for every three hours until 21h.
创建时间:
2021-10-06



