Figure S1 - Fusogenic Pairings of Vesicle-Associated Membrane Proteins (VAMPs) and Plasma Membrane t-SNAREs – VAMP5 as the Exception

FACS analysis of expression levels of SNAREs at the cell surface. 24 h after cotransfection with tTA and the empty vector, flipped VAMPs 1, 3, 4, 5, 7 or 8 (v-cells), or 24 h after cotransfection with TRE-LacZ, flipped SNAP-25 and syntaxins 1 or 4 (t-cells), unpermeabilized COS-7 cells were stained with an anti-Myc antibody, and then analyzed by flow cytometry. The mean fluorescence intensity of staining of the SNAREs was determined by FACS analysis. Each plasmid was transfected at 1 mg per 10 cm2 growth area. Error bars represent standard deviation of two independent experiments.
(0.01 MB PDF)