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Oligonucleotides used for Luminex MOL-PCR.

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Figshare2015-12-02 更新2026-04-29 收录
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1Nomenclature according to [2].2Position of SNP in reference to H37Rv.3SNP in an essential gene (“ess”); synonymous SNP (“syn”).4Strand orientation of oligonucleotides.5LPO, left probe oligonucleotide, annealing upstream of SNP of interest and including it; RPO, right probe oligonucleotide, annealing downstream of SNP of interest.6xTag bead with coupled antiTag-sequence.7Universal primer sequences (upper case, not underlined), allele-specific tag-sequences (lower case), and sequences hybridizing to the template genomic sequence (underlined). RPO are 5′-phosphorylated.
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2015-12-02
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