Unusual enzymology and peptide natural products from novel Acidobacteriota spp. associated with reef-building corals and holobiont-Sulfidibacter corallicola interactions

Raw Data for characterization of RiPP BGCs from Acidobacteriota

Plasmids

Plasmid maps of constructs used for heterolgeous production of precursors and maturases in E. coli - gb format.

Aci

.raw LC-MS files of proteolytic digests (GluC) of AciA1 and AciA2 produced in the presence of AciKC or without.

Tha

.raw LC-MS files of proteolytic digests (Try or AspN) of ThaA produced in the presence of ThaM and/or ThaO or without.Subfolders include LC-MS files of 13C and 15N labeling data, Derivatisation with IAA, RaneyNi, and Marfey's reagent, and point mutants.

NMR

Raw data files for 1H (1), 1H-1H COSY (2), NOESY (3), HSQC (4), and HMBC (5) spectra of purified and modified tryptic fragment of ThaA.

MolecularNetworking

Output files generated from GNPS (one folder for the pellet network and one for the supernatant network).

CrudeExtracts

.raw LC-MS files of crude extracts from S. corallicola supernatant (SN) or pellet (P) cultured with POM or without POM (NoOM). Extracts from medium with or without POM (bu no S. corallicola) are labelled with "_neg".

RNAseq

R script and input files -- including raw feature counts, plasmidsaurus feature annotations, antiSMASH feature annotations and DESeq2 results -- used to analyze BGC transcription data of S. corallicola cultivated with and without coral-derived dissolved organic matter.

CellCounts

Raw data files, metadata, and modified cell count data from cultivating S. corallicola with and without coral-dervied dissolved organic matter.